Useful links that will often be
used in Genetics Lab:
National Center for Biotechnology Information - portal to databases and tools:
OMIM (On-line Mendelian Inheritance in Man)
Human Gene Mutation
Data, pictures of gels, etc.:
Intron-Exon borders (2004)
Restriction Mapping (2004)
Pictures of gels from Restriction mapping lab
Gel 1, photo 1 - size marker ladder looks slightly better on this one
Gel 1, photo 2 - restriction bands look slightly better on this one
Gel 2 - I run this gel after class, used a longer and denser gel for better resolution
of smaller fragments of similar size.
This gel also contains a little extra points assignment.
Lanes 1-4 (on the left of the gel) show PCR amplified mini-satellite DNA
(DNA fingerprint) from:
Lane 1 - Suspect #1
Lane 2 - Suspect #2
Lane 3 - Suspect #3
Lane 4 - Evidence (DNA collected at the crime scene).
Who is guilty as charged? Explain what each band means.